By Lemuel Wingard (Eds.)
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Extra info for Applied Biochemistry and Bioengineering. Enzyme Technology
T h e s e membranes are often made o f cellulose derivatives which are attacked by the cellulase enzymes pres ent in many fermentation broths o f fungal origin. , composite membranes based on polysulfones on a carrier fabric. T h e concentrated enzyme solution is usually turbid due to precipi tates formed in the concentration process. It also contains varying amounts o f bacteria or other microorganisms. T h e s e are removed by a germ filtration process. Because o f the small capacity o f the germ fil ters, the process must b e preceded by a polishing filtration.
This method also has drawbacks: pure maltose is difficult to obtain and maltose preparations are often contaminated by maltotriose which is hydrolyzed at a higher rate. Furthermore, glucoamylase is not used for maltose hydrolysis; hence the method cannot b e applied in comparing glucoamylase s o f Asper gillus and Rhizopus, which have different ratios between hydrolysis o f starch and maltose. T h e problems described are simple compared with the analytical difficulties o f other important enzymes, such as milk-coagulating en zymes, proteases, and pectinases.
And Long, Μ. E. (1972). S. Patent No. 3,645,848. Lilly, M. , and Dunnill, P. (1969). In "Fermentation Advances" (D. ), pp. 225-237. Academic Press, New York. Lilly, M. , and Dunnill, P. (1971). Process Biochem. August, 29. Luedeking, R. (1967). In "Biochemical and Biological Engineering Science" (N. ), Vol. 1, pp. 181-243. Academic Press, New York. MacLeod, C. J. , and Lilly, M. D. /. Gen. Microbiol. 89, 221. Malby, P. G. (1970). , August, 22. Matteo, C. , and Demain, A. L. (1975). Biotechnol.
Applied Biochemistry and Bioengineering. Enzyme Technology by Lemuel Wingard (Eds.)