Animal Cell Culture - download pdf or read online

By Mohamed Al-Rubeai (eds.)

ISBN-10: 3319103199

ISBN-13: 9783319103198

ISBN-10: 3319103202

ISBN-13: 9783319103204

​Animal cells are the popular “cell factories” for the creation of complicated molecules and antibodies to be used as prophylactics, therapeutics or diagnostics. Animal cells are required for the right kind post-translational processing (including glycosylation) of biopharmaceutical protein items. they're used for the creation of viral vectors for gene treatment. significant ambitions for this remedy contain melanoma, HIV, arthritis, cardiovascular and CNS illnesses and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological reviews. This ebook is designed to function a finished assessment of animal mobile tradition, overlaying the present prestige of either learn and purposes. For the coed or R&D scientist or new researcher the protocols are imperative to the functionality of telephone tradition paintings, but a wide realizing is vital for translation of laboratory findings into the economic construction. in the large scope of the publication, each one subject is reviewed authoritatively through specialists within the box to supply state of the art choice of present study. a tremendous reference quantity on phone tradition learn and the way it affects on construction of biopharmaceutical proteins around the world, the e-book is key studying for everybody operating in cellphone tradition and is a instructed quantity for all biotechnology libraries.

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Biotechnol Bioeng 106:774–783 Matrai J, Chuah MK, Vandendriessche T (2010) Recent advances in lentiviral vector development and applications. Mol Ther 18:477–490 Miller DG, Wang PR, Petek LM, Hirata RK, Sands MS, Russell DW (2006) Gene targeting in vivo by adeno-associated virus vectors. Nat Biotechnol 24:1022–1026 Miller JC, Holmes MC, Wang J, Guschin DY, Lee YL, Rupniewski I, Beausejour CM, Waite AJ, Wang NS, Kim KA, Gregory PD, Pabo CO, Rebar EJ (2007) An improved zinc-finger nuclease architecture for highly specific genome editing.

This is also reflected by a wide range of commercial reagents available for this application. Different lipid-based transfection reagents have been studied in comparative studies (also with reagents from the group of cationic polymers). The performance was shown to depend highly on the cell line used for transfection as well as other process parameters (Schlaeger et al. 2003; Rosser et al. 2005; Haldankar et al.

Hauser Capecchi MR (2005) Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century. Nat Rev Genet 6:507–512 Carlson CM, Dupuy AJ, Fritz S, Roberg-Perez KJ, Fletcher CF, Largaespada DA (2003) Transposon mutagenesis of the mouse germline. Genetics 165:243–256 Carrondo M, Panet A, Wirth D, Coroadinha AS, Cruz P, Falk H, Schucht R, Dupont F, GenyFiamma C, Merten OW, Hauser H (2011) Integrated strategy for the production of therapeutic retroviral vectors. Hum Gene Ther 22:370–379 Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF (2011) Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting.

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Animal Cell Culture by Mohamed Al-Rubeai (eds.)


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